Recent Publications

Exosomes and their Therapeutic Applications. 2016.

Yeo R.W.Y. and Lim S.K.

ADVANCES IN PHARMACEUTICAL CELL THERAPY: Principles of Cell-Based Biopharmaceuticals. 477-501

Mesenchymal stem cell exosomes. 2015.

Lai R.C., Yeo R.W.Y. and Lim S.K.

Seminars in Cell & Developmental Biology. 40: 82-88.

Focus on Extracellular Vesicles: Therapeutic Potential of Stem Cell-Derived Extracellular Vesicles 2016.

Zhang B., Yeo R.W.Y., Tan K.H. and Lim S.K.

International Journal of Molecular Sciences. 17(2): 174

Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper. 2015.

Lener T., Gioma M., Aigner L., Börger V., Buzas E., Camussi G., Chaput N., Chatterjee D., Court F.A. and del Partillo H.A.

Journal of Extracellular Vesicles. 4: 30087

The diagnostic and prognostic potential of plasma extracellular vesicles for cardiovascular disease. 2015.

Bank I.E.M., Timmers L., Gijsberts C.M., Zhang Y., Mosterd A., Wang J., Chan M.Y., de Hoog V., Lim S.K. and Sze S.K.

Expert Review of Molecular Diagnostics. 15: 1577-1588.


Plasma biomarker discovery in preeclampsia using a novel differential isolation technology for circulating extracellular vesicles.

Hian Tan K1, Sim Tan S2, Sze SK3, Ryan Lee WK4, Jack Ng M4, Kiang Lim S

Am J Obstet Gynecol. 2014 Mar 18 [Epub ahead of print]

Efficiency of Exosome Production Correlates Inversely with the Developmental Maturity of MSC Donor

Tian Sheng Chen, Ronne Wee Yeh Yeo, Fatih Arslan, Yijun Yin, Soon Sim Tan, Ruenn Chai Lai, Andre Choo, Jayanthi Padmanabhan, Chuen Neng Lee, Dominique P.V. de Kleijn, Kok Hian Tan, Sai Kiang Lim Journal of Stem Cell Research & Therapy 2013, 3:3.


Exosome: A Novel and Safer Therapeutic Refinement of Mesenchymal Stem Cell

Ronne Wee Yeh Yeo, Ruenn Chai Lai, Kok Hian Tan, Sai Kiang Lim.

Exosomes and Microvesicles, Vol. 1, 7:2013. The Journal of the American Society for Exosomes and Microvesicles. Accepted 13 Nov 2013.


Derivation and Characterization of Human Fetal MSCs: an alternative cell source for large-scale production of cardioprotective microparticles.

Lai RC, Arslan F, Tan SS, Tan B, Choo A, Lee MM, Chen TS, Teh BJ, Eng JK, Sidik H, Tanavde V, Hwang WS, Lee CN, El Oakley RM, Pasterkamp G, de Kleijn DP, Tan KH, Lim SK.

J Mol Cell Cardiol. 2010 Jun;48(6):1215-24.


Exosome for Drug Delivery – A Novel Application for Mesenchymal Stem Cell.

Lai RC, Yeo RW, Tan KH, Lim SK.

Biotechnol Adv. 2013 Sep-Oct;31(5):543-51.


MSC exosome ameliorates reperfusion injury through a proteomic complementation.

Lai RC, Yeo RW, Tan KH, Lim SK.

Regen Med. 2013 Mar;8(2):197-209.


Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury.

Lai, R.C., Arslan, F., Lee, M.M., Sze S.K., Choo, A., Chen, T.S., Salto-Tellez, M., Timmers, L., Lee, C.N., El Oakley, R.M., Pasterkamp, G., de Kleijn, D.P.V., Lim S.-K. (2010).

Stem Cell Research 4: 214-222.


Mesenchymal stem cell secretes microparticles enriched in pre-microRNAs.

Chen T.S., Lai, R.C., Lee, M.M., Choo, A., Lee, C.N., Lim, S.K.* (2010).

Nucleic Acid Research 38 (1): 215–224.


Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs.

Chen, T.S., Arslan, F., Yin Y., Tan, S.S., Lai, R.C., Choo, A., Padmanabhand, J., Lee C.N., de Kleijn, D.P.V., and  Lim S.-K.* (2011).

Journal of Translational Medicine 9:47-57.


Mesenchymal stem cell exosome: a novel stem cell-based therapy for cardiovascular disease.

Lai, R.C., Chen, T.S. and Lim S.-K.* (2011).

Regen. Med. 6(4), 481–492.


Mesenchymal Stem Cell Exosomes: The Future MSC-Based Therapy?

Lai, R., Yeo, R., Tan, S., Zhang, B., Yin, Y., Sze, S., Choo, A., and Lim, S. (2013).

In Mesenchymal Stem Cell Therapy, L.G.V. Chase, Mohan C. , ed. (Humana Press), pp. 39-62. ISBN 978-1-62703-200-1.


Mesenchymal stem cell: an efficient mass producer of exosomes for drug delivery.

Yeo W.Y.R. Lai, R.C., Zhang B, Tan SS, Yin Y, Teh BJ and Lim S.-K.* (2013).

Advanced Drug Delivery Review 65 336–341.


Mesenchymal stem cell exosome ameliorates reperfusion injury through proteomic complementation.

RC Lai, RWY Yeo, KH Tan, SK Lim (2013).

Regenerative Medicine 8 (2), 197-209.


Exosomes for drug delivery – a novel application for the mesenchymal stem cell.

Lai, R.C., Yeo W.Y.R., Tan K.H. and Lim S.-K.*.

Biotechnology Advances (2012 Aug 25. [Epub ahead of print]).


Measurement of precursor miRNA in exosomes from human ESC-derived mesenchymal stem cells.

Chen, T.S., and Lim S.-K.*.

Book Chapter:Methods in Molecular Biology: Circulating MicroRNAs: Methods and Protocols.  ISBN 978-1-62703-452-4.


Microvesicle protein levels are associated with increased risk for future vascular events and mortality in patients with clinically manifest vascular disease.

Kanhai DA, Visseren FLJ, van der Graaf, Y, Schoneveld AH, Catanzariti L, Timmers L, Kappelle J, Uiterwaal C, Lim SK, Sze, SK, Pasterkamp G, de Kleijn DP.

Int. J. Cardiol. 2013 Feb 25. doi:pii: S0167-5273(13)00291-X. 10.1016/j.ijcard.2013.01.231. [Epub ahead of print]


Serum extracellular vesicle protein levels are associated with acute coronary syndrome.

de Hoog VC, Timmers L,  Schoneveld AH, Wang JW, van de Weg SM, Sze, SK, van Keulen JK,  Hoes AW,  den Ruijter HM de Kleijn DP, Mosterd A.

Eur. Heart J.: Acute Cardiov. Care. 2013, 2(1) 53–60


Mesenchymal stemcell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury.

Arslan F, Lai RC, Smeets MB, Akeroyd L, Choo A, Aguor EN, Timmers L, van Rijen HV, Doevendans PA, Pasterkamp G, Lim SK, de Kleijn DP.

Stem Cell Res. 2013 May;10(3):301-12.


Proteolytic Potential of the MSC Exosome Proteome: Implications for an Exosome-Mediated Delivery of Therapeutic Proteasome.

Lai RC, Tan SS, Teh BJ, Sze SK, Arslan F, de Kleijn DP, Choo A, Lim SK.

Int J Proteomics. 2012;2012:971907.


Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease.

van Koppen A, Joles JA, van Balkom BW, Lim SK, de Kleijn D, Giles RH, Verhaar MC.

PLoS One. 2012;7(6):e38746.